1.目的:保持菌株的原始特征和活力尽可能稳定,以确保菌株不会死亡,未被充实并且不会受到外部污染的污染。菌株的出色特征不会退化和优异菌株的存活。2。原理:通过减少微生物的代谢,抑制生长和繁殖,延长菌株的生命并减少突变。常见方法是低温,低氧和干燥。云茶国际交易怎么盈利
1.储存细菌的目的
1。目的
2.原理
代理商和缺乏营养也可以改善保存效果。
2.节省细菌的方法是什么?
1.光温保存方法
将细菌接种到适当的固体斜率培养基中。细菌完全生长后,用油纸带一棉,然后将其转移至2-8°C,然后将其存储在冰箱中。
2.线石蜡保存方法
(1)用三角形将一瓶液体石蜡煮沸,用棉塞,用牛皮纸将绷带插入,0.1mpa,在121.3°C下灭菌约30分钟,然后转移到40分钟的°C蒸水箱中。
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(2)在斜坡培养基中培养细菌,获得浓的细菌,然后使用灭菌的稻草将石蜡注入斜坡。
(3) Stay the test tube upright and save it at low temperature or room temperature.
3. Filter paper preservation method
(1) Prepare the paper strip (0) .5 × 1.2cm), Anxuan tube (0.6 × 8cm), put 1-2 photos into each tube, and then insert the plug into 0.1MPa, sterilize 121.3 ℃, sterilize at 121.3 ℃30 minutes.
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(2) Cultivate bacteria in the oblique medium, obtain a strong bacteria, and then take out 1-2ml sterilized and skimmed milk, drip in a sterilized dine, and then use cyclocular mossSuspension.
(3) Pluck the filter strip with a mule, soak it in the bacterial suspension, put it back to the pipe tube after suction, and plug it with a cotton plug.
(4) Put the pipe tube into a dryer equipped with a pentagonal dioxide, use a vacuum pump pump to dry, and then pump into the cotton into the tube, melt it with flames, and save it at low temperature.
(5) When using the bacteria, heated the mouth of the Anxian tube on the flame, drip a drop of cold water at the heating site, break the glass, and then knock off the glass at the bottle mouth with a mule.After opening the pipe, take out the filter paper, put it in the liquid medium, and put it in the thermal box to cultivate.
Fourth, sand preservation method
(1) Take out the river sand, add 10%dilute hydrochloric acid, heat and boil for 30 minutes (remove organic matter).
(2) Pour the acid water out, wash the river sand into neutral with tap water, and then dry it, sieve (40 mesh sieve) to remove thick particles.
(3) Take thin loquat or red soil that does not contain corrupt implantation, soak and wash several times until neutral.
(4) Dry, crushed, sieved (100 mesh sieve) to remove coarse particles.
(5) Mix according to the ratio of 2: 1, put it in the test tube, plug it in cotton plug, sterilize for about 30 minutes.
(6) Cultivate bacteria in the oblique medium, obtain a strong bacteria, and then use sterilized water to clean the cells or spores into the mi of 3-5 into the oblique culture.
(7)使用无菌吸管吸收细菌悬浮液,均匀地滴入砂管,每根管道0.2-0.5ml,然后在管道中取出水。如果是螺纹细菌和霉菌,则可以将孢子直接捡入沙管。
(8)用火焰的火焰的梅尔时间,然后将它们放在干燥的地方(4-6°C)中,或用牛皮或塑料纸将砂管直接包裹,然后将其存放在烘干机中。检查每六个月的存储过程,细菌的存活和纯度。
5.甘油管保存方法
(1)高压蒸汽灭菌剂80%甘油。
(2)在倾斜培养基中培养细菌,然后用无菌水进行高浓度的细菌悬浮液。
(3)与1ML甘油和细菌液均匀混合,使得甘油的浓度约为10-30%,然后放置在减去70%C的冰点环境中。
(4)甘油 - 生理盐水(体积比8:2)也可以与细菌肉汤均匀混合,然后在减去20°C的环境中储存。
6.冷冻和干燥的保存方法
(1)在同一时间,在低温下快速冷冻细胞,然后在真空状态下干燥,以确保在冷冻和水升华过程中不会损坏细胞,应使用保护剂来准备细胞悬浮液(可溶于在冷冻和脱水过程中保护效果的可溶性),氢键和离子键用于产生水和细胞以产生水和细胞。亲和力的形状稳定了细胞成分。
(2)将干细菌封装在真空下以隔离空气以实现长期存储。
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(3)该方法的主要因素是保留低温,干燥和氧气除湿。除了电线形的菌丝体外,大多数其他微生物都适用。
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